For the first time since the abduction of 84-year-old Nancy Guthrie, Savannah Guthrie’s sister and brother-in-law have been spotted looking tense.

Annie Guthrie, aged 56, and her husband, Tomasso Cioni, were seen leaving their Tucson, Arizona home in a dark Honda SUV around 5 p.m. MT on Tuesday, attempting to shield themselves from view.

Annie tried to obscure her face from the cameras by covering her head with a coral-colored hoodie.

Cioni used his t-shirt to cover his mouth and nose, complementing his disguise with a pair of aviator sunglasses to avoid being photographed.

The couple, who wed in 2006 and share a child, briefly returned to their $650,000 Tucson residence shortly thereafter.

Pima County Sheriff Chris Nanos noted that they were the last known individuals to have seen Nancy before she disappeared.

Savannah is believed to be staying with them at her home. 

FBI agents visited the property around 2:30pm MT on Tuesday as they continue to investigate the kidnapping of Savannah and Annie’s mother Nancy. They left later the same afternoon.

Annie Guthrie and her husband Tomasso Cioni cover their faces as they leave their Tucson home on Tuesday afternoon

Guthrie and Tomasso were the last people to see Nancy Guthrie before her abduction late Saturday night or early Sunday morning

The couple were photographed at their $650,000 Tucson home, where Savannah is also believed to be staying 

Nancy was taken from her $1 million Tucson home sometime between Saturday night and Sunday morning, with investigators saying her kidnapper had forced entry. 

Annie and her mother had spent the evening enjoying a dinner together and playing Mahjong – a Chinese four-player rummy style game.

Pima County Sheriff Chris Nanos has not offered any information about a possible suspect or suspects, with a former FBI profiler telling the Daily Mail on Tuesday that Nancy was likely selected for abduction ‘in advance.’  

Annie raised the alarm the following morning after Nancy failed to show up for church.

Ransom notes were delivered to TMZ and local news outlet KOLD on Tuesday afternoon demanding an undisclosed sum of Bitcoin.

The notes are said to have shared details about damage to Nancy’s home and what she was wearing that have not been released to the public. 

Pima County Sheriff’s Office have acknowledged them but have refused to verify their contents, or confirm whether their deputies have received any ransom demands for Nancy. 

Savannah Guthrie and her missing mother Nancy are pictured together. Two ransom notes were sent to news outlets on Tuesday, but it is unclear if they are genuine 

Annie Guthrie and Tomasso Cioni are pictured together in a social media snap. The couple have been married since 2006 and have one child 

The FBI are helping with the case and President Trump himself has offered assistance. 

A $2,500 reward has been offered for information about her disappearance.  

Footage shared on Monday showed blood drops on the doorstep of Nancy’s home. 

She requires regular medication for a health condition and investigators have warned that denying Nancy these could result in her death.

Police sources told Fox News on Tuesday that Nancy’s pacemaker stopped transmitting information to her Apple Watch around 2am on Sunday.

The Apple Watch was found in Nancy’s home, suggesting she had been taken out of its range when her pacemaker stopped sending data. 

At Tuesday’s press conference, Sheriff Nanos was unable to offer information about a possible suspect or suspects.

He said his deputies were still trying to obtain footage from Nancy’s doorbell camera.

It was missing from its holder when investigators arrived. 

FBI agents were pictured arriving at the home of Annie and Tomasso on Tuesday. Savannah is also believed to be at the property

Federal agents are seen outside the property. They are now helping with the hunt for Nancy

Blood spots are seen on the doorstep of Nancy’s $1 million Tucson home after her abduction

The doorbell camera holder sits empty. Sheriff Chris Nanos says his deputies are still trying to access footage from its server 

Nancy Guthrie’s $1 million home is pictured. She has lived at the property since 1975 

Nanos said there were no tire tracks at the scene but insisted he was still hoping to bring Nancy home safe and well.

She has lived at the property since 1975. 

Her husband Charles died of a heart attack in 1988 aged just 49, leaving Nancy to raise Savannah, Annie and her son Camron as a single mother. 

On Monday night, Nanos welled-up when asked by a reporter if he thought Nancy was still alive and conceded the window in which she would realistically be found unharmed was closing.

Savannah has not appeared on NBC’s Today since the abduction. 

The show has led with the story every day since but its reporters admitted Wednesday there was little new information to share with viewers. 

Savannah issued a statement on Monday night requesting prayers. She has dropped out of hosting the Milan Winter Olympics while the hunt for her mother continues.